DMEM (Dulbecco’s Modified Eagle Medium) is a widely used cell culture medium designed to support the growth of a variety of mammalian cells. It contains essential nutrients, such as amino acids, vitamins, glucose, and salts, along with varying concentrations of calcium and magnesium. DMEM can be supplemented with fetal bovine serum (FBS) and other growth factors to promote optimal cell proliferation and maintenance in culture.